A Biochemical Dissection of the RNA Interference Pathway in Drosophila melanogaster: A Dissertation
نویسندگان
چکیده
xii CHAPTER I: INTRODUCTION Overview and history of RNAi Transcriptional silencing: mechanisms and phylogeny Small RNAs and translational repression Self-regulation of small RNA-mediated pathways Small RNA biogenesis RNAi effector proteins RNA-silencing pathway functions RNAi and disease Concerns regarding RNAi-based therapy CHAPTER II: ATP REQUIREMENTS AND SMALL INTERFERING RNA STRUCTURE IN THE RNA INTERFERENCE PATHWAY Summary Introduction Results and discussion ATP and dsRNA processing siRNA duplexes are bona fide intermediates in the RNAi pathway A second ATP-dependent step in RNAi Target recognition and cleavage are ATPindependent siRNA-protein complex formation siRNA unwinding during the RNAi reaction 5' phosphorylation status and siRNA activity An ATP-dependent step downstream of siRNA unwinding A model for the RNAi pathway Materials and methods
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